Journal: Cell reports

Article Title: Herpesvirus-induced spermidine synthesis and eIF5A hypusination for viral episomal maintenance

doi: 10.1016/j.celrep.2022.111234

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human: Endothelial cells immortalized with hTERT (TIME) , ATCC , CRL-4025.

Techniques: Virus, Recombinant, Membrane, cDNA Synthesis, SYBR Green Assay, Purification, Mass Spectrometry, Plasmid Preparation, Software

Journal: Free radical biology & medicine

Article Title: Circulating CD36 is increased in hyperlipidemic mice: cellular sources and triggers of release

doi: 10.1016/j.freeradbiomed.2021.03.004

Figure Lengend Snippet: (A) Primary human dermal microvascular endothelial cells (HDMVECn) were incubated with 20μM oxPCCD36 (HODA-PC) or control POPC for 6h and CD36 was detected in the media and cell lysate by Western blot analysis. (B) Human platelets were isolated from platelet rich plasma by gel filtration and incubated with oxPCCD36 (HODA-PC) and control POPC. Supernatant and lysate were analyzed for CD36 by Western blot analysis. (C) 3T3L1 preadipocytes were differentiated to adipocytes and then incubated with 20μM oxPCCD36 (HODA-PC) or control POPC for 6h and CD36 was detected in the media and cell lysate by Western blot analysis. Actin is used as a loading control for cellular CD36. Right side panels show the quantification data of band intensity (mean ± SD of at least 3 independent experiments). The quantification data of band intensity shown in bar graph (n≥5). * p<0.05, ** p<0.01, *** p<0.001, and ns p>0.05.

Article Snippet: Primary Human Dermal Microvascular Endothelial Cells; Normal, Neonatal (HDMVECn) cells were purchased from ATCC and cultured as recommended.

Techniques: Incubation, Control, Western Blot, Isolation, Clinical Proteomics, Filtration